Further New Components in the Volatile Oil of Sage

Abstract Background: As mentioned in the first part of this study, sage leaves and their essential oils are very important and most commonly used in the food, drug and perfumery indus-tries. The volatile oil of dried leaves of "Sage" Salvia Offic-inalis was obtained by hydrodistillation method (1.2% v/w). Aim of Study: The present study was a continuation of the previous one where four new constituents Kuwaitine 1, 2, 3, 4 were unveiled further analysis was carried out along the same procedure to determine and evaluate the essential components of the volatile oil of sage by (GC-MS) provided further data exposing further constituents. Material and Methods: Crushed dried Leaves (100g), were subjected to hydrodistillation E.P. (1984) GC-MS analysis was performed on Hewlett Packard 5850GC CONNECTED TO AN HP 5970 quadruple nano spectrometer (70cw) and an HP 9000 work station with helium as the carrier gas. Results: As mentioned in part one of the study 26 com-ponents representing 95% of the total oil were identified. Four of these components were not reported before. Thujone, Camphor Camphene, Pinene, Myrecene, Eucalyptol, Terpinlen, Linolool, Borneol, a-Terpinol and most abundant constituents of the oil about (51%). In addition significant amounts of unreported constituents were found to be present such as Peak No. 19, 20, 23, 40 whose Mwt and suggested stu preliminary chemical structure were elucidated and called Kuwaitene 5, 6, 7, 8 by the Author. Conclusion: GC-MS analysis of the volatile oil revealed at least 35 components, most of which could be identified, four of which were not reported in the literature before (peak no. 19, 20, 23 and 40. M. Wts 136 (Bicyclo 4. 1.0 HEPTANE, 7 (I-methylethylidene) 196 (2-Nor bornanol), 196 Isomer (Terpinyl acetate), 332 (Sandaracopimar-15, en-8, beta-yl acetate) respectively) named by the author as "Kuwaitene 5, 6, 7, 8".