Effect of Leukocyte Counts in Seminal Plasma on DNA Fragmentation of Spermatozoa

OMAIMA KHAMISS, Ph.D.1, DALIA M. MOSTAFA, Ph.D.4; MANAL EL-HAMSHARY, Ph.D.2; AHMED M. BAHAA ELDIN, M.D.3 And

doi:10.21608/mjcu.2024.411328

Effect of Leukocyte Counts in Seminal Plasma on DNA Fragmentation of Spermatozoa

Document Type : Original Article

Author

DALIA M. MOSTAFA, Ph.D.4; MANAL EL-HAMSHARY, Ph.D.2; AHMED M. BAHAA ELDIN, M.D.3 and OMAIMA KHAMISS, Ph.D.1

Cell Culture and Molecular Virology, Genetic Engineering & Biotechnology Research Institute, University of Sadat City1 Molecular Diagnostic Department, Genetic Engineering & Biotechnology Research Institute, University of Sadat City2 Obstetrics & Gynecology Department, Faculty of Medicine, Ain Shams University3 and Obstetrics & Gynecology Department, Faculty of Medicine, Ain Shams University and M.Sc. Candidate in Reproduction Technologies Genetic Engineering and Biotechnology Research Institute, University of Sadat City4

10.21608/mjcu.2024.411328

Abstract

Abstract Background: Infections of the male genitourinary tract account for up to 15% of cases of male infertility. Leukocy-tospermia is the presence of leukocytes in the semen, and the presence of >1 million leukocytes per mL of semen is consid-ered as pathological. Aim of Study: To investigate the effect of leukocyte count on DNA fragmentation percent. Patients and Methods: The study included one hundred male participants from Zagazig City, Sharkiya, Governorate, Egypt in El-Banoon fertility Center from February 2019 to Feb-ruary 2023. Patients were divided into two groups: Group I con-trol group consisting of 50 healthy males with normal semen analysis and Group II consisting of 50 males had leukocytes in the semen >1 million per mL. Results: There was no significant difference between the 2 groups as regard age and BMI. Percentages of viscous sam-ples and semen liquefactiontime had significantly higher and Percentages of progressive and total sperm motility had signifi-cantly lower in study group as compared to control group. leu-kocyte concentration and DNA Fragmentation were significant-ly higher in study group than control group while Viability was significantly lower in study group in comparison with control group. The study group had significantly lower sperm dynam-ic motility parameters including VCL, VSL, VAP, MAD, ALH and STR as compared to the control group. BCF and WOB were significantly higher with study group indicating a nega-tive effect on linearity. Conclusion: The present study suggested that bacterial in-fection significantly correlated with leukocytospermia, decreas-ing sperm motility, morphology, and DNA integrity.

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