Prevalence of Ochrobactrum Intermedium in Gastric Biopsy Specimens of Patients with Gastritis and Dyspepsia and the Impact of Amniotic Membrane as Antibacterial

MEDHAT HASHEM, Ph.D.4; ASHRAF EID, Ph.D.5; AHMED SEIF, Ph.D.5 And OMAIMA KHAMISS, Ph.D.6, WAFAA ELSHABSHIRY, M.Sc.1; RATEB ABBAS, Ph.D.2; ELHAM AWAD, Ph.D.3;

doi:10.21608/mjcu.2024.411639

Prevalence of Ochrobactrum Intermedium in Gastric Biopsy Specimens of Patients with Gastritis and Dyspepsia and the Impact of Amniotic Membrane as Antibacterial

Document Type : Original Article

Author

WAFAA ELSHABSHIRY, M.Sc.1; RATEB ABBAS, Ph.D.2; ELHAM AWAD, Ph.D.3; MEDHAT HASHEM, Ph.D.4; ASHRAF EID, Ph.D.5; AHMED SEIF, Ph.D.5 and OMAIMA KHAMISS, Ph.D.6

1-Animal Cell and Tissue Culture, Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute University of Sadat City, 2-Professor of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute University of Sadat City, Egypt. 3-Assistant Professor of Medical Microbiology and Immunology, Shebin El-Kom Teaching Hospital, Shebin El-Kom, Egypt 4-Professor of Genetics, Genetic Engineering and Biotechnology Research Institute University of Sadat City, Egypt. 5-Professor of Gastroenterology, Hepatology and Infectious Diseases, Shebin El-Kom Teaching Hospital, Shebin El-Kom, Egypt 6-Professor of Cell Culture and Molecular Virology, Genetic Engineering and Biotechnology Research Institute University of Sadat City, Egypt

10.21608/mjcu.2024.411639

Abstract

Abstract Background: Ochrobactrum intermedium bacteria has some roles as an opportunistic organism in many cases of gas-tritis and dyspepsia. Human amniotic membrane is known to have an antimicrobial effect on several organisms. Aim of Study: This study briefly aims to screening for a novel anti-bacterial treatment on some pathogenic gastrointes-tinal tract bacteria. Material and Methods: Sixty-three gastric biopsies from dyspeptic patients were collectedfrom (Shebin El-Kom Teach-ing Hospital, Shebin El-Kom, Egypt). Selected isolate of O.in-termedium from thirty positive isolates was molecular identi-fied and phylogenic tree was carried out using 16S rRNA which was sequenced and compared with available 16S ribosomal se-quences in the NCBI GenBank database (OR067155). Human Amniotic membrane (HAM) was freshly isolated from the pla-centa and HAECs have been enzymatically isolated using pre-warm 0.05% trypsin/EDTA, then cells were seeded in standard culture medium DMEM/F12 (Gibco, Thermo Fisher Scientific, USA) supplemented with 10% FBS and incubated at 37ºC. Cell counts was done using a hemocytometer, cell maintenance and viability was followed-up using trypan blue. Possible inhibi-tory activity of HAM against O.intermedium has been tested and recorded. Ochrobactrum genus have been confirmed in the human gastric niche based on PCR. Results: Biopsies from patients with gastroesophageal re-flux disease showed H.pylori existence in histopathology with also positive urease, catalase and oxidase tests, while PCR re-sults showed O.intermedium without H.pylori. Human amniot-ic membrane had an inhibitory effect against O.intermedium. Conclusion: This study demonstrates that Human amniotic membrane can be used as an antibacterial against O.intermedi-um in cases of gastritis or dyspepsia, especially in immunocom-promised patients, O.intermedium should be considered as an opportunistic organism whether H.pylori exists or not.

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