MicroRNA29-a as Diabetic Nephropathy Biomarker in Diabetic Patients (Type 2)

SALWA E. MOHAMED, M.D., MOHAMED Y. NASR, M.D.;; RANDA A. GHANOUM, M.D., AHMED M. HASSANAIN, M.D.;; EL-ABED, M.D., SABAH F.

doi:10.21608/mjcu.2019.54875

MicroRNA29-a as Diabetic Nephropathy Biomarker in Diabetic Patients (Type 2)

Document Type : Original Article

Authors

The Department of Molecular Biology, Genetic Engineering & Biotechnology Institut, Sadaat City University* and Laboratory Department, National Heart Institute, General Organization of Hospitals & Teaching Institutes, Ministry of Healthy

10.21608/mjcu.2019.54875

Abstract

Abstract
Background: Diabetic nephropathy (DN) is one of the main causes of renal failure. Recent research demonstrated that the serial microbes (miRNA) could be used as a marker to detect pathogenic disease; the DN-related miRNAs could not be studied.
Aim of Study: In this study the aim was to investigate the predictive value of miRNA29-a in diabetes mellitus type 2 (T2DM) patients in relation to nephropathy risk onset predic-tion.
Subjects and Methods: In the present study a total of (120) persons were divided into three groups as Group (I): (N=20) control group, Group (II): (N=50) T2DM patients without renal symptoms Group (III): (N=50) T2DM patients with renal insufficiency, RT-PCR method was used to determine the relative quantitative of miRNA29-a.
Results: Increased levels of HbA1c, FBS, urea, creatinine and Albumin/creatinine ratio (ACR) were observed in subjects under study.An increased levels of miRNA 29-a in group III, DN (51.97-}61.78) and group II, DM (7.06±6.83) when com-pared with group I, control subjects (0.8-}0.2). The result of the present study implied that miRNA 29-a could be served as an early indicator of DM-mediated renal pathogenesis.

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