Effect of Triazolopyridine (Trapidil) on Experimental Hepatorenal Toxicity Induced by Renal Ischemia Reperfusion Injury in Rats

Abstract
Background: Renal ischemia, followed by reperfusion (I/R) is one of the major causes of Acute Renal Failure (ARF) furthermore it resulted in distant organ dysfunctions. The pathogenesis of I/R is multifactorial.
This study was designed to study the effect of trapidil treatment before renal I/R on hepatic and renal function,
Material and Method: 30 male Sprague-Dawley rats were submitted to right nephrectomy and divided into three groups: Control group, renal I/R group, renal I/R group received trapidil 14mg/kg 7 days before I/R, 24 hours after reperfusion blood samples were collected for evaluation of: Serum Aspar-tate Aminotransferase (AST), serum alanine aminotransferase (ALT), blood urea nitrogen, serum creatinine, serum Nitric Oxide (NO), Tumor Necrosis Factor-a (TNF-a), Total Anti-oxidant Capacity (TAC), and serum Monocyte Chemoattractant Protein-1 (MCP-1). All animals were scarified, the liver and left kidney were removed to measure renal and hepatic Malond-idhyde (MDA), Glutathione Peroxidase (GPx) and Myeloper-oxidase (MPO), histopathological examination for the left kidneys was performed.
Results: Treatment with trapidil (14mg/kg) for 7 days before renal I/R improved renal and hepatic dysfunction as evidenced by significant decrease in blood urea, serum creat-inine, and significant decrease in the activity of AST, ALT as compared to renal I/R group in addition trapidil supplemen-tation resulted in significant decrease in serum, TNF-a, MCP-1 as compared to renal I/R group on the other hand it resulted in significant increase in serum NO and total antioxidant capacity in addition it resulted in significant decrease in the level of renal and hepatic tissue MPO and MDA with signif-icant increase in tissue GPx as compared to renal I/R group these results are confirmed by histopathological findings.
Conclusion: Trapidil supplementation before renal I/R can protect the kidney and liver and prevent its damage by increasing NO production with improvement of blood flow, anti-inflammatory and antioxidant effect.